การประชุมวิชาการระดับชาติ ครั้งที่ 16
มหาวิทยาลัยราชภัฏนครปฐม

          This research aimed to isolate actinomycetes capable of producing pectinase enzyme from three orchard soils in Sampran district, Nakhon Pathom province, Thailand. The effectiveness of pectin degradation on solid pectin agar plates incubated at room temperature for 5 days was also investigated.

Results revealed the successful isolation of 200 actinomycete isolates from the three orchard soils. Upon screening for pectin degradation ability, 81 isolates exhibited clear zones on the solid agar plates, representing 40.5% of the total isolates tested. The majority of these pectinolytic isolates (50 isolates, representing 25%) exhibited clear zone diameters between 1.00 and 2.99 cm. The remaining isolates showed clear zone diameters of 2.00-2.99 cm (21 isolates, 10.5%), 3.00-3.99 cm (9 isolates, 4.5%), and 0.1-0.99 cm (1 isolate, 0.5%). Notably, 119 isolates (59.5%) failed to produce pectinase enzyme. Among the pectinolytic isolates, isolate SM4 demonstrated the largest clear zone diameter of 4.05 cm, followed by isolates CHI4 (3.7 cm), SM4.2 (3.65 cm), and CHI8 (3.6 cm).