Antimicrobial activities of banana peel extract observed in this research could contribute to the future production of new value-added antimicrobial products from banana peels which are considered as agricultural wastes. Hence, the purpose of this research was to study the antibacterial effects of the extracts from Musa (AAA group) peel. Banana peel extraction was performed using two different solvents, 95% ethanol and 100% methanol, and investigated their abilities to inhibit three strains of the following bacteria: Escherichia coli ATCC 25922, Salmonella Typhimurium ATCC 13311 and Staphylococcus aureus ATCC 25923. Our results showed that ethanolic extract of Musa (AAA group) banana peel had the ability to inhibit Staphylococcus aureus ATCC 25923 with the inhibition zone diameter of 13.50 ± 1.29 mm while methanolic extract of Musa (AAA group) peel did not show antibacterial effects against all of the three strains of bacteria. Determination of the minimum inhibitory concentration (MIC) of the extract that could inhibit the growth of Staphylococcus aureus ATCC 25923 by macro broth dilution techniques followed by the investigation of minimum bactericidal concentration (MBC) on the MHA medium showed the values of MIC and MBC at 10 and 20 mg/ml, respectively.