The purposes of the research were to 1) develop and find the quality of online lesson cooperate with 5E approach in science subjects, 2) find the efficiency of online lesson cooperate with 5E approach, 3) compare a learning achievement before and after studied with online lesson cooperate with 5E approach, and 4) study the satisfaction of students on studied with online lesson cooperate with 5E approach. The target group consisted of 21 students in grade 6 students at Ban Don Phai School (Ratrangsan). The tools used in this research were the learning plans, the online lesson, the pretest/post-test, the exercise, the content assessment form, the technique and methodology assessment form, and the satisfaction questionnaire. The data were analyzed by pertcentage, mean, standard deviation, and dependent t-test.

            The research findings showed that 1) online lesson cooperate with 5E in science subjects had 3 units, the quality of the content, and the quality of the technique and methodology were at the highest level, 2) online lesson had efficiency 82.06/80.63 according to the criteria of 80/80, 3) the learning achievement of students after studied with online lesson cooperate with 5E was higher than before studied with the statistical significance level at .01, and 4) the students’ satisfaction on studied with online lesson cooperate with  5E was at the highest level.