This research develops an automatic alcohol-based disinfection dispenser with a microcontroller. It is to 1) design and develop a movable automatic alcohol-based disinfection dispenser with a microcontroller, 2) evaluate its performance. The system will work by measuring the temperature of the user whether the person exceeds the set temperature of 37.5 degrees Celsius. If the temperature exceeds the set temperature, an alert will be created. As for the people with normal temperature, they can go through the gate for an automatic sterilization process. The system consists of three parts, including, 1) a palm thermometer, 2) an alcohol-disinfection dispenser, and 3) a gauge level measurement tool for the alcohol. The first part is to measure the temperature by hand using a microcontroller (Arduino Mega 2560) and a temperature sensor (Infrared Temperature Sensor Module). The measured temperature is displayed on a 4-digit 7-segment LED display. The second part, the alcohol-disinfection dispenser, is to spray the alcohol disinfection responding to people who walk through the gate. The infrared Photoelectric switch count sensor will activate the system when it detects someone passing through. Finally, the last part, the alcohol gauge level measurement tool is to monitor the quantity of the alcohol solution left in the system. The system will alert when the solution has dropped to a certain amount which is set to 1,500 milliliters. The results show that all three parts of the system work 100% correctly.