During the COVID-19 pandemic, village health volunteers (VHVs) played an important role in preventing the spread of diseases in the community. This can lead to work-related stress. The objective of this descriptive study was to study the factors related to stress among village health volunteers in the COVID-19 situation. The study population was 43 village health volunteers at Sisathong Sub-district, Nakhon Chaisi District, Nakhon Pathom Province. The research instrument was the stress-related factors questionnaire consisting of 5 parts: 1) personal factors, 2) work-related factors, 3) perception of COVID-19, 4) concern on COVID-19 and 5) ST5 stress questionnaire. The results found that most of the village health volunteers were female (88.4%), 61-70 years old of age (44.2%), primary education (53.5%), ≤ 3 years of experience (25.6%), and 1-2 working days per week (46.5 %). The overall role performance in disease control was a high level (3.96±0.39). The overall interpersonal relationship was at the highest level (4.40±0.45). The overall perception of COVID-19 was the highest level (4.27±0.39). The overall concern on COVID-19 was a high level (3.77±0.77). Most participants have a low-stress level (83.7%) followed by moderate stress level (14.0%) and high-stress level (2.3%), respectively. Moreover, there was no relationship between personal factors, work-related factors, perception of COVID-19, and concern on COVID-19 factor with the stress of village health volunteers.