The objective of this research was to select suitable solvents for extracting pigments from frozen mulberries (Morus alba L.). Four types of solvents were used, namely distilled water, 100% methanol, 95% ethanol, and 20% acetic acid at a ratio of 1:1 (weight g)/ solution volume (ml)). Those solvents were tested for chromosomal staining efficiency of onion root tips, it was found that methanol frozen mulberry extract had the highest mean of satisfaction with a satisfaction level of 4.51. The extract was then treated with a hot air oven drying process and ground to powder. After that, the mulberry powder was dissolved with methanol at the ratio of 1:2 and tested for chromosome staining at the tip of the onion root. The extract can still stain the chromosomes clearly and can keep the powder color for about 1 month.