Phosphorus (P) is an important element in growth and development of plant. It had role in root development, flower formation and production of yield. The objective of this research was to study the effect of phosphorus fertilizer on growth, yield, P uptake and partitioning of P in different parts of dwarf yard long bean. The experimental design was carried out in 2x2 factorial in CRD, which the first factor was dwarf yard long bean varieties such as KKU25 and CLGC30 and the second factor was no P fertilizer (-P) and P fertilizer at recommended rate (24 kg. P₂O₅/rai) (+P). The results showed that the application of P fertilizer was increased yield significant difference. The partitioning of P in plant found that the distribution of P had the highest value in pod tissue with the range from 46.3-50.2 percent, followed by leave, root and stem respectively. The variety of CLGC30 had total biomass (104 g/plant) higher than KKU25 (65.7 g/plant). In addition, the variety of CLGC30 had also total P uptake (553 mg/plant) higher than KKU25 (357 mg/plant) and the application of P fertilizer (474 mg/plant) provided the higher of P uptake than no P fertilizer treatment (436 mg/plant).