The surveying of microbial diversity at Phu Toei National Park Suphanburi, soil samples were collected at 0-15 cm depth from 5 areas; Pa Sak (PS), Tad Huai Bamboo Forest (PR), Dry Bamboo Forest (PL), Dwarf Forest (PT) ,and Moss Bamboo Forest (PM). Then, microorganisms were isolated by dilution plate method using Tryptone Soya Agar (TSA) for microorganisms, National Botanical Research Institute's Phosphate (NBRIP) ,and Pikovskaya agar (PVK) for phosphate solubilizing microorganisms. The results indicated that the microorganisms were found in all of the soil sampling areas. The results showed the highest number of microorganisms on Tryptone Soya Agar, followed by phosphate solubilizing microorganisms in a significant difference (p ≤ 0.05). And the highest number of microorganisms was in the Dry Bamboo Forest in a significant difference (p ≤ 0.05). Fungi were isolated on Potato Dextrose Agar (PDA) and Rose Bengal Agar (RBA). The fungi were found in all of the soil sampling areas. The results showed that the number of fungi on Potato Dextrose Agar is higher than Rose Bengal Agar, And the highest number of fungi was in Pa Sak and Dwarf Forest with a significant difference (p ≤ 0.05). The results of this study found that microbial diversity in each area was different and found different 11 bacterial isolates and 22 fungus isolates in order to the studies are to test the properties and to take advantage.