This research is focused the study of isolation and potential of bacterial separation from the rubber processing to improve the wastewater efficiency. The bacterial isolates from soil, rubber industry wastewater treatment influent and sludge. From the study, the effectiveness of 3 strains for wastewater treatment were Streptomyces atrovirens, Arthrobacter sp. and Streptomyces sp. All three strains can be applied for increasing the efficiency of the wastewater treatment system from the rubber processing.